Physiology & Pharmacology
Ensieh Namgou; Roya Lari; Naser Mahdavi Shahri; Ali Moghimi; Mehrnegar Rojhan Nezhad; Leila Izi
Volume 25, Issue 1 , May and June 2018, , Pages 93-100
Abstract
Background and purpose: granulocyte colony-stimulating factor (G-CSF) has a major role in blood cells differentiation especially in neutrophils, therefore G-CSF widely has been used in treatment of chemotherapy-induced neutropenia. Different lines of evidence indicated that G-CSF is increased in inflammatory ...
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Background and purpose: granulocyte colony-stimulating factor (G-CSF) has a major role in blood cells differentiation especially in neutrophils, therefore G-CSF widely has been used in treatment of chemotherapy-induced neutropenia. Different lines of evidence indicated that G-CSF is increased in inflammatory situation and has inflammatory characteristics. Unlike G-CSF, type I interferones (IFNs) have been shown to have anti-inflammatory effects. The aim of this study was to investigate whether type I IFNs can normalize the effects of G-CSF on blood factors. Materials and methods: forty-two mice (male, eight weeks) were divided into six groups of seven, which includes Group 1 (control) injection of distilled water, Group 2 injection 200 µg/kg b.w. G-CSF, Group 3 injection 200 µg/kg b.w. Alfa interferon (IFN-α), Group 4 injection 200 µg/kg b.w. interferon beta (IFN-β), Group 5 injection 200 µg/kg b.w. G-CSF+200 µg/kg b.w. IFN-α, Group 6 injection 200 µg/kg b.w. G-CSF+200 µg/kg b.w. IFN-β. After 28 days, blood was taken from each mouse heart and blood biochemical parameters (Glucose, Triglyceride, Cholestrol) was investigated. Results: surprisingly, in most cases, the G-CSF and type one IFNs alone or simultaneously reduced the levels of Glucose, triglycerides and cholesterol. Also the reduction of glucose, triglycerides and cholesterol were higher in G-CSF and INFs in compare with G-CSF alone. Our data raise the possibility that, G-CSF and INF-α/β have some similarity in signaling pathway which has to be investigated.
Masoud Fereydoni; Maliheh Eskandari; Ali Moghimi
Volume 20, Issue 4 , January and February 2014, , Pages 423-434
Abstract
Introduction: Morphine has been known as a drug with different paradoxical effects, analgesic and hyperalgesic. On the other hand, repeated morphine administration, induces morphine tolerance in mammals. The aim of recent study is investigating tolerance to morphine in Drosophila melanogaster and the ...
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Introduction: Morphine has been known as a drug with different paradoxical effects, analgesic and hyperalgesic. On the other hand, repeated morphine administration, induces morphine tolerance in mammals. The aim of recent study is investigating tolerance to morphine in Drosophila melanogaster and the effect of Oseltamivir (an inhibitor of G2 receptors) on hyperalgesia induced by low concentrations of morphine and morphine tolerance.
Materials and methods: In this study, stage 3 of larvae and adult state of wild Drosophila melanogaster were used. For evaluating the effect of Oseltamivir on hyperalgesic effect of low concentration Morphine, Oseltamivir (0.2 mg/l) and low concentrations of morphine were added to media culture. Then behavior of larvae and adults to thermal (using Hot plate, 47˚C) and chemical (capsaicin and acetic acid) pain stimulations were recorded. For morphine tolerance experiments in larvae, repeated morphine administration (0.1 and 0.01 mg/l) was done and their response to thermal pain was evaluated. The same method was used in adults but with other doses of morphine (200 and 300 mg/l). Finally to investigate the mechanism of morphine tolerance, Oseltamivir was administered too.
Results: morphine tolerance was occurred in Drosophila melanogaster similar to mammals. Repeated morphine administration diminished anti nociceptive effects of morphine (p
Ali Moghimi; Mohammad Mohammad-Zadeh; Seyyed Mehdi Beheshti Nasr
Volume 19, Issue 1 , March and April 2012, , Pages 14-25
Abstract
Background: Minocycline is an antibiotic and anti-inflammatory drug. In addition, its neuroprotective effects have been shown. Since there is interaction between cell death and seizure, the aim of this study is examination of the role of minocycline on amygdala-kindled seizures in rat.
Materials and ...
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Background: Minocycline is an antibiotic and anti-inflammatory drug. In addition, its neuroprotective effects have been shown. Since there is interaction between cell death and seizure, the aim of this study is examination of the role of minocycline on amygdala-kindled seizures in rat.
Materials and Methods: In this experimental study, three groups of animals (18 rats), after stereotaxic surgery and 1-week recovery period, received twice daily kindling stimulations. In fully kindled animals of groups 1-3, minocycline was injected intraperitoneally in doses 12.5 (n = 7), 25 (n = 5) and 50 (n = 6) mg/kg, respectively, 60 minutes before stimulation. Afterdischarge duration (ADD), stage 4 latency (S4L), Stage 5 Duration (S5D) and Seizure Duration (SD) were recorded and compared with related control groups (the same animals that had received saline 1 day before). A p-value of less than 0.05 was considered to represent a significant difference.
Results: In fully kindled animals who had received minocycline (50 and 25 mg/kg), ADD decreased significantly. When minocycline was delivered, S5D decreased 38.3% (p < 0.001), 34% (p